Coding

Part:BBa_M50025:Design

Designed by: James Benjamin Hu   Group: Stanford BIOE44 - S11   (2016-10-27)


GolS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 421
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

While the GolS gene was originally drawn from York iGEM 2013, we made a modification to the start codon. We changed the start codon to -AUG- to increase the efficiency of translation in E. coli. This is because E. coli more frequently uses AUG to initiate translation.


Source

This part was taken from the 2013 York iGEM group: https://parts.igem.org/Part:BBa_K1127008

References